Plant Transcription Factor Database
Previous version: v3.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT3G17609.2
Common NameBZIP64, HYH, MKP6.27
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family bZIP
Protein Properties Length: 149aa    MW: 16898.7 Da    PI: 9.1272
Description HY5-homolog
Gene Model
Gene Model ID Type Source Coding Sequence
AT3G17609.2genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
       bZIP_1   2 kelkrerrkqkNReAArrsRqRKkaeieeLeekvkeLeaeNkaLkkeleelkkevaklks 61 
                  ke +  +r+ +NR++A+  R+RKk ++  Le ++ eL++ N++L +++++l++e   l++
                  778889***********************************************9987765 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SMARTSM003387.6E-1376140IPR004827Basic-leucine zipper domain
PfamPF001701.2E-1277136IPR004827Basic-leucine zipper domain
PROSITE profilePS5021711.56578141IPR004827Basic-leucine zipper domain
SuperFamilySSF579591.38E-1380138No hitNo description
CDDcd147044.46E-1582131No hitNo description
Gene3DG3DSA: hitNo description
PROSITE patternPS0003608398IPR004827Basic-leucine zipper domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009585Biological Processred, far-red light phototransduction
GO:0010224Biological Processresponse to UV-B
GO:0080167Biological Processresponse to karrikin
GO:0005634Cellular Componentnucleus
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005515Molecular Functionprotein binding
GO:0043565Molecular Functionsequence-specific DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009025anatomyvascular leaf
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 149 aa     Download sequence    Send to blast
Expression -- Microarray ? help Back to Top
Source ID E-value
Expression AtlasAT3G17609-
Functional Description ? help Back to Top
Source Description
TAIREncodes a homolog of HY5 (HYH). Involved in phyB signaling pathway.
UniProtTranscription factor that promotes photomorphogenesis in light. Acts downstream of the light receptor network and directly affects transcription of light-induced genes. Specifically involved in the blue light specific pathway, suggesting that it participates in transmission of cryptochromes (CRY1 and CRY2) signals to downstream responses. In darkness, its degradation prevents the activation of light-induced genes. {ECO:0000269|PubMed:12023303}.
Function -- GeneRIF ? help Back to Top
  1. The data support the hypothesis that the opposite root system phenotypes of hy5 single and hy5 hyh double mutants represent the morphological response to a quantitative gradient in the same molecular process.
    [PMID: 17121469]
  2. important for high nitrate reductase expression in red light
    [PMID: 17929051]
  3. This research provides evidence that, in ultraviolet B (UV-B) signaling, UVR8, HY5, and HYH act together and demonstrates a new role for HYH in UV-B responses.
    [PMID: 18055587]
  4. HYH and PEX11b constitute a novel branch of the phyA-mediated light signaling cascade, which promotes peroxisome proliferation during seedling photomorphogenesis.
    [PMID: 18203870]
  5. HY5 and HYH were activators of NIA2, but inhibitors of NRT1.1 when tested across various light treatments and tissue types.
    [PMID: 19540016]
  6. HY5 and HYH are two necessary regulators that play a pivotal role during low temperature-induced anthocyanin accumulation in Arabidopsis seedlings.
    [PMID: 20932601]
  7. Together with HY5/HYH, gibberellins signaling may play an important role during low temperature-induced anthocyanin accumulation.
    [PMID: 21636970]
  8. light triggers MSBP1 expression through direct binding to and activation by HY5 and HYH.
    [PMID: 21715650]
  9. the functional interrelations of GBF1 with HY5 and HYH in Arabidopsis seedling development.
    [PMID: 22692212]
  10. Data indicate that the PIF1/PIF3-HY5/HYH transcriptional modules mediate crosstalk between light and ROS signaling and a mechanism by which plants adapt to the light environments.
    [PMID: 23645630]
  11. BBX24 and BBX25 physically interact with HYH.
    [PMID: 23733077]
  12. The DNA binding activity of GBF1 is modulated by heterodimeriation with HY5 and HYH.
    [PMID: 24157608]
  13. This study further reveals that MYC2 and GBF1 bind to HYH promoter and inhibit each other through non-DNA binding bHLH-bZIP heterodimers.
    [PMID: 26047210]
Cis-element ? help Back to Top
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT1G12110(R), AT1G12370(A), AT1G37130(A), AT3G22840(A), AT3G47430(A), AT5G13930(A)
Interaction ? help Back to Top
Source Intact With
BioGRIDAT4G36730, AT5G11260, AT1G09530
IntActSearch Q8W191
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT3G17609
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAF4534770.0AF453477.1 Arabidopsis thaliana HY5-like protein (HYH) mRNA, complete cds.
GenBankAK1754400.0AK175440.1 Arabidopsis thaliana mRNA for bZip transcription factor AtbZip64, complete cds, clone: RAFL21-87-C16.
GenBankAK1755230.0AK175523.1 Arabidopsis thaliana mRNA for bZip transcription factor AtbZip64, complete cds, clone: RAFL21-94-F18.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_850605.11e-105transcription factor HY5-like protein
SwissprotQ8W1911e-107HYH_ARATH; Transcription factor HY5-like
TrEMBLD7L7A35e-93D7L7A3_ARALL; Putative uncharacterized protein
STRINGAT3G17609.21e-105(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Representative plantOGRP20811737
Publications ? help Back to Top
  1. Jakoby M, et al.
    bZIP transcription factors in Arabidopsis.
    Trends Plant Sci., 2002. 7(3): p. 106-11
  2. Holm M,Ma LG,Qu LJ,Deng XW
    Two interacting bZIP proteins are direct targets of COP1-mediated control of light-dependent gene expression in Arabidopsis.
    Genes Dev., 2002. 16(10): p. 1247-59
  3. Subramanian C, et al.
    A suite of tools and application notes for in vivo protein interaction assays using bioluminescence resonance energy transfer (BRET).
    Plant J., 2006. 48(1): p. 138-52
  4. Sibout R, et al.
    Opposite root growth phenotypes of hy5 versus hy5 hyh mutants correlate with increased constitutive auxin signaling.
    PLoS Genet., 2006. 2(11): p. e202
  5. Vandenbussche F, et al.
    HY5 is a point of convergence between cryptochrome and cytokinin signalling pathways in Arabidopsis thaliana.
    Plant J., 2007. 49(3): p. 428-41
  6. Jonassen EM,Lea US,Lillo C
    HY5 and HYH are positive regulators of nitrate reductase in seedlings and rosette stage plants.
    Planta, 2008. 227(3): p. 559-64
  7. Brown BA,Jenkins GI
    UV-B signaling pathways with different fluence-rate response profiles are distinguished in mature Arabidopsis leaf tissue by requirement for UVR8, HY5, and HYH.
    Plant Physiol., 2008. 146(2): p. 576-88
  8. Desai M,Hu J
    Light induces peroxisome proliferation in Arabidopsis seedlings through the photoreceptor phytochrome A, the transcription factor HY5 HOMOLOG, and the peroxisomal protein PEROXIN11b.
    Plant Physiol., 2008. 146(3): p. 1117-27
  9. Safrany J, et al.
    Identification of a novel cis-regulatory element for UV-B-induced transcription in Arabidopsis.
    Plant J., 2008. 54(3): p. 402-14
  10. Kumagai T, et al.
    The common function of a novel subfamily of B-Box zinc finger proteins with reference to circadian-associated events in Arabidopsis thaliana.
    Biosci. Biotechnol. Biochem., 2008. 72(6): p. 1539-49
  11. Lillo C
    Signalling cascades integrating light-enhanced nitrate metabolism.
    Biochem. J., 2008. 415(1): p. 11-9
  12. Datta S, et al.
    LZF1/SALT TOLERANCE HOMOLOG3, an Arabidopsis B-box protein involved in light-dependent development and gene expression, undergoes COP1-mediated ubiquitination.
    Plant Cell, 2008. 20(9): p. 2324-38
  13. Jonassen EM,Sévin DC,Lillo C
    The bZIP transcription factors HY5 and HYH are positive regulators of the main nitrate reductase gene in Arabidopsis leaves, NIA2, but negative regulators of the nitrate uptake gene NRT1.1.
    J. Plant Physiol., 2009. 166(18): p. 2071-6
  14. Sellaro R,Hoecker U,Yanovsky M,Chory J,Casal JJ
    Synergism of red and blue light in the control of Arabidopsis gene expression and development.
    Curr. Biol., 2009. 19(14): p. 1216-20
  15. Hu J,Desai M
    Light control of peroxisome proliferation during Arabidopsis photomorphogenesis.
    Plant Signal Behav, 2008. 3(10): p. 801-3
  16. Kaur N,Hu J
    Dynamics of peroxisome abundance: a tale of division and proliferation.
    Curr. Opin. Plant Biol., 2009. 12(6): p. 781-8
  17. Gong W, et al.
    The development of protein microarrays and their applications in DNA-protein and protein-protein interaction analyses of Arabidopsis transcription factors.
    Mol Plant, 2008. 1(1): p. 27-41
  18. Rubin G,Tohge T,Matsuda F,Saito K,Scheible WR
    Members of the LBD family of transcription factors repress anthocyanin synthesis and affect additional nitrogen responses in Arabidopsis.
    Plant Cell, 2009. 21(11): p. 3567-84
  19. Charron JB,He H,Elling AA,Deng XW
    Dynamic landscapes of four histone modifications during deetiolation in Arabidopsis.
    Plant Cell, 2009. 21(12): p. 3732-48
  20. Jonassen EM,Sandsmark BA,Lillo C
    Unique status of NIA2 in nitrate assimilation: NIA2 expression is promoted by HY5/HYH and inhibited by PIF4.
    Plant Signal Behav, 2009. 4(11): p. 1084-6
  21. Nelson DC, et al.
    Karrikins enhance light responses during germination and seedling development in Arabidopsis thaliana.
    Proc. Natl. Acad. Sci. U.S.A., 2010. 107(15): p. 7095-100
  22. Castells E, et al.
    det1-1-induced UV-C hyposensitivity through UVR3 and PHR1 photolyase gene over-expression.
    Plant J., 2010. 63(3): p. 392-404
  23. Aung K,Zhang X,Hu J
    Peroxisome division and proliferation in plants.
    Biochem. Soc. Trans., 2010. 38(3): p. 817-22
  24. Zhang Y,Zheng S,Liu Z,Wang L,Bi Y
    Both HY5 and HYH are necessary regulators for low temperature-induced anthocyanin accumulation in Arabidopsis seedlings.
    J. Plant Physiol., 2011. 168(4): p. 367-74
  25. Fehér B, et al.
    Functional interaction of the circadian clock and UV RESISTANCE LOCUS 8-controlled UV-B signaling pathways in Arabidopsis thaliana.
    Plant J., 2011. 67(1): p. 37-48
  26. Zhang Y,Liu Z,Liu R,Hao H,Bi Y
    Gibberellins negatively regulate low temperature-induced anthocyanin accumulation in a HY5/HYH-dependent manner.
    Plant Signal Behav, 2011. 6(5): p. 632-4
  27. Shi QM,Yang X,Song L,Xue HW
    Arabidopsis MSBP1 is activated by HY5 and HYH and is involved in photomorphogenesis and brassinosteroid sensitivity regulation.
    Mol Plant, 2011. 4(6): p. 1092-104
  28. Lang-Mladek C, et al.
    UV-B signaling pathways and fluence rate dependent transcriptional regulation of ARIADNE12.
    Physiol Plant, 2012. 145(4): p. 527-39
  29. Singh A,Ram H,Abbas N,Chattopadhyay S
    Molecular interactions of GBF1 with HY5 and HYH proteins during light-mediated seedling development in Arabidopsis thaliana.
    J. Biol. Chem., 2012. 287(31): p. 25995-6009
  30. Xie L,Hauser MT
    Induction of ARI12 upon broad band UV-B radiation is suppressed by UVR8 and cryptochromes.
    Plant Signal Behav, 2012. 7(11): p. 1411-4
  31. Ram H,Chattopadhyay S
    Molecular interaction of bZIP domains of GBF1, HY5 and HYH in Arabidopsis seedling development.
    Plant Signal Behav, 2013. 8(1): p. e22703
  32. Chen D, et al.
    Antagonistic basic helix-loop-helix/bZIP transcription factors form transcriptional modules that integrate light and reactive oxygen species signaling in Arabidopsis.
    Plant Cell, 2013. 25(5): p. 1657-73
  33. Tilbrook K, et al.
    The UVR8 UV-B Photoreceptor: Perception, Signaling and Response.
    Arabidopsis Book, 2013. 11: p. e0164
  34. Ram H,Priya P,Jain M,Chattopadhyay S
    Genome-wide DNA binding of GBF1 is modulated by its heterodimerizing protein partners, HY5 and HYH.
    Mol Plant, 2014. 7(2): p. 448-51
  35. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
  36. Maurya JP,Sethi V,Gangappa SN,Gupta N,Chattopadhyay S
    Interaction of MYC2 and GBF1 results in functional antagonism in blue light-mediated Arabidopsis seedling development.
    Plant J., 2015. 83(3): p. 439-50